Composite

Part:BBa_K5507009:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


sacB-5-Ptac-kana-rrnB-T-sacB-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 785
    Illegal BglII site found at 2203
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1947


Design Notes

The part includes sacB-5, tac promoter, kana gene, rrnB-T terminator, sacB-3. sacB-5 and sacB-3 are the flanking sequences of the sacB gene. After transforming this component into K. xylinus, the bacteria will gain resistance to kanamycin. Subsequently, introducing BBa_K5507008 into the bacteria will lead to the loss of kanamycin resistance, resulting in the pgi overexpression (OE) strain. When constructing the pgi-OE gdh-KO strain, transforming BBa_K5507010 into the pgi OE strain will restore the kanamycin resistance gene. In summary, the construction of this component serves as a selection marker to verify the success of the genetic modifications.


Source

The part is Composite parts.

References